An enzymatic cycling method for the determination of ammonia.
نویسندگان
چکیده
منابع مشابه
Carnitine determination by an enzymatic cycling method with carnitine dehydrogenase.
We describe a highly sensitive and specific method for determining L-carnitine in serum by use of carnitine dehydrogenase (EC 1.1.1.108). The method involves a new enzymatic cycling technique with NADH, thio-NAD+, and carnitine dehydrogenase, and measures the increase of absorbance at 415 nm of thio-NADH produced at 37 degrees C during the reaction: [formula: see text] The calibration curve for...
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* Present address, Department of Biochemistry, University of California, Berkeley 4, California. 1 The following abbreviations have been used; THF, tetrahydrofolic acid; lo-formyl-THF, N*o-formyltetrahydrofolic acid; 5,10-methenyl-THF, the cyclic N6-NrOimidazolinium derivative of 5-formyl-THF (anhydroleucovorin or anhydrocitrovorum factor) ; ATP, adenosine triphosphate; ADP, adenosine diphospha...
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ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 1989
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.38.4_188